A method of direct measurement for the enzymatic determination of cholesteryl esters

被引：35

作者：

Mizoguchi, T ^{[1
]}

Edano, T ^{[1
]}

Koshi, T ^{[1
]}

机构：

[1] Kowa Co Ltd, Div Pharmaceut, Tokyo New Drug Res Labs 2, Tokyo 1890022, Japan

来源：

JOURNAL OF LIPID RESEARCH | 2004年 / 45卷 / 02期

关键词：

cholesterol assay; cholesterol oxidase catalase; plasma; liver; tissue culture;

D O I：

10.1194/jlr.D300024-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A direct measurement method for the enzymatic determination of cholesteryl esters (CEs) without measuring total cholesterol (TC) and free cholesterol (FC) is described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE was measured by enzymatic determination using a colorimetric method or a fluorometric method. The measurement sensitivity of the fluorometric method was more than 20 times that of the colorimetric method. Optimal conditions of the assay were determined, and examples of measured CE in human plasma, rat liver, and cultured cells are indicated. The method of directly measuring CE was simple and has exceptional reproducibility compared with the technique of subtracting FC from TC using each measured TC and FC.

引用

页码：396 / 401

页数：6