Isolation and characterization of a bovine gene encoding phenolsulfotransferase

被引：4

作者：

Henry, T

Kliewer, B

Palmatier, R

Ulphani, JS

Beckmann, JD

机构：

[1] UNIV NEBRASKA,MED CTR,DEPT INTERNAL MED,SECT PULM & CRIT CARE,OMAHA,NE 68198

[2] UNIV NEBRASKA,MED CTR,DEPT BIOCHEM & MOL BIOL,OMAHA,NE 68198

来源：

GENE | 1996年 / 174卷 / 02期

关键词：

gene structure; recombinant DNA; nucleotide sequence; gene expression; transfection; kinetics;

D O I：

10.1016/0378-1119(96)00083-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Phenol sulfotransferases (PST) esterify hydroxylated aromatic compounds with sulfate, and therefore play a role in the metabolism of xenobiotics. In this investigation, a bovine PST gene of 2372 bp was amplified from genomic DNA. Sequence overlap with the cognate cDNA revealed seven exons, with all introns containing GT/AG splicing donor/acceptor sites. This product was subcloned into an SV40-based expression vector and transfected into WI-26 human lung fibroblasts. Immunoblot analyses revealed production of the anticipated 32-kDa protein, and the active enzyme displayed steady-state kinetic properties consistent with the enzyme characterized in bovine lung (apparent K-m = 6.5 mu M for 2-naphthol).

引用

页码：221 / 224

页数：4

共 20 条

[1] HUMAN THERMOLABILE PHENOL SULFOTRANSFERASE GENE (STM) - MOLECULAR-CLONING AND STRUCTURAL CHARACTERIZATION [J].