Molecularly imprinted ligand-exchange recognition assay of glucose by quartz crystal microbalance

被引:71
作者
Ersöz, A
Denizli, A
Özcan, A
Say, R [1 ]
机构
[1] Anadolu Univ, Dept Chem, Eskisehir, Turkey
[2] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey
[3] Anadolu Univ, BIBAM, Plant Drug & Sci Res Ctr, Eskisehir, Turkey
关键词
molecular imprinting; MIP; QCM; glucose sensor; ligand exchange;
D O I
10.1016/j.bios.2004.07.030
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Molecular imprinted polymers (MIP) as a recognition element for sensors are increasingly of interest and MIP-quartz crystal microbalance (QCM) have started to appear in the literature. In this study, we have combined quartz crystal microbalance with MIP to prepare a sensor using the ability of glucose to chelate of copper (II) ion of methacrylamidohistidine (MAH) monomer to create ligand exchange (LE) assembled monolayer which is suitable for glucose determination. The study includes the measurement of binding interaction of molecularly imprinted QCM sensor via ligand interaction, investigation of the pH effect on frequency shift and recognition selectivity studies of glucose-imprinted polymer with respect to methyl-alpha-(D)-glueopyranoside and sucrose. B-max (number of binding sites) and K-D (dissociation constant of the metal-chelate copolymer) were also calculated using Scathard plot and the detection limit was found as 0.07 mM. MIP showed higher glucose-binding affinity than a well-known glucose binding protein, conconavalin A. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:2197 / 2202
页数:6
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