Precursor-directed polyketide biosynthesis in Escherichia coli

被引：18

作者：

Kinoshita, K

Pfeifer, BA

Khosla, C

Cane, DE

机构：

[1] Brown Univ, Dept Chem, Providence, RI 02912 USA

[2] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem & Biochem, Stanford, CA 94305 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2003年 / 13卷 / 21期

关键词：

D O I：

10.1016/j.bmcl.2003.08.008

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates. (C) 2003 Elsevier Ltd. All rights reserved.

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页码：3701 / 3704

页数：4

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