Precursor-directed polyketide biosynthesis in Escherichia coli

被引：18

作者：

Kinoshita, K

Pfeifer, BA

Khosla, C

Cane, DE

机构：

[1] Brown Univ, Dept Chem, Providence, RI 02912 USA

[2] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem & Biochem, Stanford, CA 94305 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2003年 / 13卷 / 21期

关键词：

D O I：

10.1016/j.bmcl.2003.08.008

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：3701 / 3704

页数：4

共 13 条

[11] Biosynthesis of complex polyketides in a metabolically engineered strain of E-coli [J].