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An integrated microfluidic device for influenza and other genetic analyses

被引：172
作者：
Pal, R
Yang, M
Lin, R
Johnson, BN
Srivastava, N
Razzacki, SZ
Chomistek, KJ
Heldsinger, DC
Haque, RM
Ugaz, VM
Thwar, PK
Chen, Z
Alfano, K
Yim, MB
Krishnan, M
Fuller, AO
Larson, RG
Burke, DT
Burns, MA [1 ]
机构：
[1] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Elect Engn & Comp Sci, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA
[5] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
来源：
LAB ON A CHIP | 2005年 / 5卷 / 10期
关键词：
D O I：
10.1039/b505994a
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.
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收藏
页码：1024 / 1032
页数：9
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