Analysis of metallothioneins by the modified Brdicka procedure

被引：86

作者：

Raspor, B ^{[1
]}

Paic, M ^{[1
]}

Erk, M ^{[1
]}

机构：

[1] Rudjer Boskovic Inst, Ctr Marine & Environm Res, HR-10002 Zagreb, Croatia

来源：

TALANTA | 2001年 / 55卷 / 01期

关键词：

Brdicka reaction; calibration straight lines; metallothionein determination; the effect of physico-chemical parameters;

D O I：

10.1016/S0039-9140(01)00399-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Quantitative analysis of metallothioneins (MTs) by the modified Brdicka procedure is based on the evaluation of the catalytic hydrogen signal (Cat). The effect of the basic physico-chemical parameters, the temperature and the depolarizer concentration (Co(NH3),Cl-3), on the Cat signal height and the respective calibration straight lines was studied by means of the differential pulse (DP) mode at the hanging mercury drop electrode (HMDE). The temperature was varied from 7 to 18 degreesC, and the depolarizer concentration from 1.2 x 10(-4) to 1.0 x 10(-3) M Co(NH3)(6)Cl-3. The commercially available rabbit liver NIT was used as the calibrant. The parameters of the calibration straight lines, the standard errors of the intercept s(a) and the slope s(b) were evaluated by means of the least-square method. The hypothesis of a significant difference of the estimated value of the intercept on y-axis compared to the zero value was statistically tested. For those conditions for which this hypothesis was rejected the measuring conditions of the catalytic hydrogen signal are selected and they refer to a buffer 1M (NH4Cl + NH4OH), pH 9.5, (7.0 +/- 0.5)degreesC and the depolarizer concentration (0.8 or 1.0) x 10(-3) M Co(NH3)(6)Cl-3. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：109 / 115

页数：7

共 18 条

[1] Concentrations of metals and metallothioneins in Mytilus galloprovincialis along the south coast of Portugal [J].

Bebianno, MJ ;

Machado, LM .

MARINE POLLUTION BULLETIN, 1997, 34 (08) :666-671

[2] Metallothionein-like metalloproteins in the Baltic clam Macoma balthica: seasonal variations and induction upon metal exposure [J].

Bordin, G ;

McCourt, J ;

Raposo, FC ;

Rodriguez, AR .

MARINE BIOLOGY, 1997, 129 (03) :453-463

[3]

BRDICKA R., 1965, TALANTA, V12, P1149, DOI 10.1016/0039-9140(65)80221-1

[4]

Brdicka R., 1933, COLLECT CZECH CHEM C, V5, P112, DOI [DOI 10.1135/CCCC19330112, 10.1135/cccc19330112]

[5]

Cosson RP, 2000, CELL MOL BIOL, V46, P295

[6] Comparison between isolation protocols commonly used for the purification of mollusc metallothioneins [J].

Geret, F ;

Rainglet, F ;

Cosson, RP .

MARINE ENVIRONMENTAL RESEARCH, 1998, 46 (1-5) :545-550

[7]

Kehr P. F., 1973, THESIS PURDUE U W LA, P97

[8]

LITEANU C, 1980, STATISTICAL THEORY M, P156

[9] ELECTROCHEMICAL APPROACH TO QUANTITATION AND CHARACTERIZATION OF METALLOTHIONEINS [J].

OLAFSON, RW ;

SIM, RG .

ANALYTICAL BIOCHEMISTRY, 1979, 100 (02) :343-351

[10] INCREASED HEPATIC METALLOTHIONEIN CONTENT CORRELATES TO CADMIUM ACCUMULATION IN ENVIRONMENTALLY EXPOSED PERCH (PERCA-FLUVIATILIS) [J].

OLSSON, PE ;

HAUX, C .

AQUATIC TOXICOLOGY, 1986, 9 (4-5) :231-242

← 1 2 →