Carbamoylation of azorhizobial nod factors is mediated by NodU

被引:11
作者
D'Haeze, W
Van Montagu, M
Promé, JC
Holsters, M
机构
[1] State Univ Ghent VIB, Dept Genet, Genet Lab, B-9000 Ghent, Belgium
[2] CNRS, Lab Pharmacol & Toxicol Fondamentales Spectrometr, F-31077 Toulouse, France
关键词
D O I
10.1094/MPMI.1999.12.1.68
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipochitooligosaccharides (LCOs) synthesized by Azorhizobium caulinodans ORS571 are substituted at the nonreducing-terminal residue with a 6-O-carbamoyl group, LCO biosynthesis in A. caulinodans is dependent on the nodABCSUIJZnoeC operon, Until now, the role of the nodulation protein NodU in the synthesis of azorhizobial LCOs remained unclear, Based on sequence similarities and structural analysis of LCOs produced by a nodU mutant, a complemented nodU mutant, and Escherichia coli DH5 alpha expressing the nodABCSU genes, NodU was shown to be involved in the carbamoylation step.
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收藏
页码:68 / 73
页数:6
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