Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages

被引:91
作者
Wuenschell, CW
Sunday, ME
Singh, G
Minoo, P
Slavkin, HC
Warburton, D
机构
[1] UNIV SO CALIF,SCH DENT,CTR CRANIOFACIAL MOLEC BIOL,LOS ANGELES,CA 90033
[2] HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115
[3] BRIGHAM & WOMENS HOSP,BOSTON,MA 02115
[4] DEPT VET AFFAIRS MED CTR,PITTSBURGH,PA
[5] UNIV PITTSBURGH,SCH MED,PITTSBURGH,PA
[6] LOS ANGELES CTY WOMENS & CHILDRENS HOSP,DIV NEONATOL,LOS ANGELES,CA
[7] UNIV SO CALIF,SCH MED,LOS ANGELES,CA
关键词
lung development; gene expression; epithelial progenitors; cell lineage; pulmonary neuroendocrine cells; Clara cells; Type II alveolar epithelial cells; immunohistochemistry; mouse;
D O I
10.1177/44.2.8609367
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Developmental expression of marker genes representative of different mature cell types can be used to study differentiation of cell lineages. We used immunohistochemistry to study expression in developing mouse lung of calcitonin gene related peptide (CGRP), Clara cell 10-KD protein (CC10), and surfactant protein-A (SP-A), markers that are differentially expressed in neuroendocrine cells, Clara cells, and Type II alveolar cells. Two distinct developmental phases were revealed, The earlier phase (embryonic days 13-15; E13-E15) was characterized by CGRP, CC10 and SP-A immunostaining in all epithelial cells of the distal airways, with the three patterns being virtually identical in adjacent sections. The later phase (E16-E18) was characterized by emergence of staining of the differentiated cell types. These expression patterns were recapitulated in serumless organ culture, demonstrating that information necessary to generate both phases of gene expression is present within the lung anlage by E11. We conclude that CGRP, CC10, and SP-A are co-expressed in most or all cells of the distal lung epithelium at E13-E15 and later become restricted to different cell lineages. This transient expression in progenitor cells of gene products characteristic of diverse differentiated cell types may reflect an underlying mechanism of gene regulation.
引用
收藏
页码:113 / 123
页数:11
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