Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain

被引：106

作者：

Tan, C

Huang, B

Chang, SF

Ngoh, GH

Munday, B

Chen, SC

Kwang, J

机构：

[1] Natl Univ Singapore, Inst Mol Agrobiol, Singapore 117604, Singapore

[2] Cent Vet Lab, Anim Hlth Serv Branch, Agrifood & Vet Author, Singapore 548596, Singapore

[3] Univ Tasmania, Sch Biomed Sci, Launceston, Tas 7250, Australia

[4] Natl Pingtung Univ Sci & Technol, Pingtung 91207, Taiwan

来源：

JOURNAL OF GENERAL VIROLOGY | 2001年 / 82卷

关键词：

D O I：

10.1099/0022-1317-82-3-647

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus (GGNNV), Singapore strain, were determined. 5'RACE and RNA ligation were used to obtain the complete nucleotide sequences of the 5' and 3' non-coding regions (NCRs), GGNNV RNA1 was determined to be 3103 nt long, containing an ORF of 982 aa, while GGNNV RNA2 was determined to be 14.33 nt long, containing an ORF of 338 aa, Both GGNNV RNAs are longer than those of other published betanodavirus sequences and the additional nucleotides were located within the NCRs. Analysis of GGNNV RNA2 revealed that it is closely related to redspotted grouper nervous necrosis virus and that both grouper viruses share the same neutralization epitope. Predicted domains for six RNA-dependent RNA polymerase motifs and two putative ORFs (proteins B1 and B2) were confirmed by sequence analysis of GGNNV RNA1.

引用

页码：647 / 653

页数：7

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