Three births after preimplantation genetic diagnosis for cystic fibrosis with sequential first and second polar body analysis

被引：26

作者：

Strom, CM ^{[1
]}

Ginsberg, N ^{[1
]}

Rechitsky, S ^{[1
]}

Cieslak, J ^{[1
]}

Ivakhenko, V ^{[1
]}

Wolf, G ^{[1
]}

Lifchez, A ^{[1
]}

Moise, J ^{[1
]}

Valle, J ^{[1
]}

Kaplan, B ^{[1
]}

White, M ^{[1
]}

Barton, J ^{[1
]}

Kuliev, A ^{[1
]}

Verlinsky, Y ^{[1
]}

机构：

[1] Illinois Masonic Med Ctr, Inst Reprod Genet, Dept Obstet & Gynecol, Chicago, IL 60657 USA

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 1998年 / 178卷 / 06期

关键词：

cystic fibrosis; preimplantation genetic diagnosis; polar bodies;

D O I：

10.1016/S0002-9378(98)70336-9

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: The purpose of this study was to determine the accuracy and feasibility of sequential polar body removal and analysis for preimplantation genetic diagnosis of mendelian disorders. STUDY DESIGN: Three couples with risk factors for cystic fibrosis had preimplantation genetic diagnosis with the use of sequential polar body analysis. After stimulation, oocytes were harvested and the first polar bodies were removed and analyzed on the day of aspiration. The following day, after fertilization, the second polar bodies were aspirated. Only embryos known to have inherited the normal maternal allele were transferred. RESULTS: All three couples had successful pregnancies resulting in the births of unaffected infants. CONCLUSIONS: Preimplantation diagnosis with the use of sequential polar body removal is feasible and can prevent the establishment of genetically abnormal pregnancies for couples at risk.

引用

页码：1298 / 1303

页数：6

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