Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR

被引：36

作者：

Conrads, G

Flemmig, TF

Seyfarth, I

Lampert, F

Lütticken, R

机构：

[1] Univ Hosp, Rhein Westfal TH Aachen, Clin Conservat & Prevent Dent & Periodontol, Inst Med Microbiol, D-52057 Aachen, Germany

[2] Univ Hosp, Rhein Westfal TH Aachen, Dept Med Microbiol, D-52057 Aachen, Germany

[3] Univ Wurzburg, Dept Periodontol, D-97070 Wurzburg, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 05期

关键词：

D O I：

10.1128/JCM.37.5.1621-1624.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.

引用

页码：1621 / 1624

页数：4

共 30 条

[1] INACTIVATION OF POLYMERASE INHIBITORS FOR MYCOBACTERIUM-TUBERCULOSIS DNA AMPLIFICATION IN SPUTUM BY USING CAPTURE RESIN [J].

AMICOSANTE, M ;

RICHELDI, L ;

TRENTI, G ;

PAONE, G ;

CAMPA, M ;

BISETTI, A ;

SALTINI, C .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :629-630

[2] Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions [J].

Ashimoto, A ;

Chen, C ;

Bakker, I ;

Slots, J .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1996, 11 (04) :266-273

[3] IMMUNOMAGNETIC PCR AND DNA-PROBE FOR DETECTION AND IDENTIFICATION OF PORPHYROMONAS-GINGIVALIS [J].

BENKIRANE, RM ;

GUILLOT, E ;

MOUTON, C .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (11) :2908-2912

[4] POLYMERASE CHAIN-REACTION FOR THE IDENTIFICATION OF PORPHYROMONAS-GINGIVALIS COLLAGENASE GENES [J].

BODINKA, A ;

SCHMIDT, H ;

HENKEL, B ;

FLEMMIG, TF ;

KLAIBER, B ;

KARCH, H .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1994, 9 (03) :161-165

[5] Live E. coli cells to treat uremia [J].

T.M.S. Chang .

Nature Medicine, 1997, 3 (1) :3-4

[6] PCR reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals [J].

Conrads, G ;

Mutters, R ;

Fischer, J ;

Brauner, A ;

Lutticken, R ;

Lampert, F .

JOURNAL OF PERIODONTOLOGY, 1996, 67 (10) :994-1003

[7] Optimized oligonucleotides for the differentiation of Prevotella intermedia and Prevotella nigrescens [J].

Conrads, G ;

Pelz, K ;

Hughes, B ;

Seyfarth, I ;

Devine, DA .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1997, 12 (02) :117-120

[8] The microbial challenge in periodontitis [J].

Darveau, RP ;

Tanner, A ;

Page, RC .

PERIODONTOLOGY 2000, 1997, 14 :12-32

[9] A ONE-STEP MICROBIAL DNA EXTRACTION METHOD USING CHELEX-100 SUITABLE FOR GENE AMPLIFICATION [J].

DELAMBALLERIE, X ;

ZANDOTTI, C ;

VIGNOLI, C ;

BOLLET, C ;

DEMICCO, P .

RESEARCH IN MICROBIOLOGY, 1992, 143 (08) :785-790

[10]

Edwards U., 1989, NUCLEIC ACIDS RES, V28, P1942

← 1 2 3 →