Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

被引：69

作者：

Bickford, Lissett ^{[1
]}

Sun, Jiantang ^{[1
]}

Fu, Kun ^{[1
,2
]}

Lewinski, Nastassja ^{[1
]}

Nammalvar, Vengadesan ^{[1
]}

Chang, Joseph ^{[1
]}

Drezek, Rebekah ^{[1
,2
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

[2] Rice Univ, Dept Elect & Comp Engn, Houston, TX 77005 USA

来源：

NANOTECHNOLOGY | 2008年 / 19卷 / 31期

关键词：

D O I：

10.1088/0957-4484/19/31/315102

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 [材料科学与工程]; 080502 [材料学];

摘要：

We demonstrate the capability of using immunotargeted gold nanoshells as contrast agents for in vitro two-photon microscopy. The two-photon luminescence properties of different-sized gold nanoshells are first validated using near-infrared excitation at 780 nm. The utility of two-photon microscopy as a tool for imaging live HER2-overexpressing breast cancer cells labeled with anti-HER2-conjugated nanoshells is then explored and imaging results are compared to normal breast cells. Five different imaging channels are simultaneously examined within the emission wavelength range of 451-644 nm. Our results indicate that under near-infrared excitation, superior contrast of SK-BR-3 cancer cells labeled with immunotargeted nanoshells occurs at an emission wavelength ranging from 590 to 644 nm. Luminescence from labeled normal breast cells and autofluorescence from unlabeled cancer and normal cells remain imperceptible under the same conditions.

引用

页数：6

共 34 条

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Quantitative evaluation of optical coherence tomography signal enhancement with gold nanoshells [J].