The auxin conjugate hydrolase family of Medicago truncatula and their expression during the interaction with two symbionts

被引:73
作者
Campanella, James J. [1 ]
Smith, Stephanie M. [1 ]
Leibu, Dora [1 ]
Wexler, Shiri [1 ]
Ludwig-Mueller, Jutta [2 ]
机构
[1] Montclair State Univ, Dept Biol & Mol Biol, Montclair, NJ 07043 USA
[2] Tech Univ Dresden, Inst Bot, D-01062 Dresden, Germany
关键词
Medicago truncatula; auxin amidohydrolases; auxin; symbiosis; arbuscular mycorrhiza; nodulation;
D O I
10.1007/s00344-007-9027-2
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have characterized the regulation of auxin conjugate hydrolysis in Medicago truncatula during development and interaction with two symbionts. In Medicago truncatula five putative auxin amidohydrolase genes (MtIAR31, MtIAR32, MtIAR33, MtIAR34, MtIAR36) were identified, homologous to the AtIAR3 gene from Arabidopsis thaliana. The MtIAR32 transcript is the most abundant transcript in whole seedlings after germination, whereas MtIAR33 is the least abundant. MtIAR32 is also the most abundant transcript in adult roots, stems, and basal leaves. MtIAR31 is most highly expressed in flowers and MtIAR36 in terminal leaves. Sinorhizobium meliloti-infected seedlings primarily upregulated MtIAR33 and -34 transcripts. Glomus intraradices-infected seedlings upregulated MtIAR33 and -34. MtIAR31, -32, -33, and -34 have hydrolytic activity against IAA-aspartate and IBA-alanine. MtIAR33, -34, and -36 hydrolyze the ester bonds of IAA-glucose. MtIAR36 solely possesses activity against IAA-glycine, -alanine, and -isoleucine. IBA was increased in Glomus intraradices-inoculated roots of Medicago compared to controls, whereas IAA was not. Our results suggest an intricate control system that regulates free and conjugated auxins in Medicago.
引用
收藏
页码:26 / 38
页数:13
相关论文
共 58 条
[11]   Isolation and characterization of mutants of Arabidopsis thaliana with increased resistance to growth inhibition by indoleacetic acid-amino acid conjugates [J].
Campanella, JJ ;
LudwigMueller, J ;
Town, CD .
PLANT PHYSIOLOGY, 1996, 112 (02) :735-745
[12]   Predicting the disulfide bonding state of cysteines with combinations of kernel machines [J].
Ceroni, A ;
Frasconi, P ;
Passerini, A ;
Vullo, A .
JOURNAL OF VLSI SIGNAL PROCESSING SYSTEMS FOR SIGNAL IMAGE AND VIDEO TECHNOLOGY, 2003, 35 (03) :287-295
[13]   His-404 and His-405 are essential for enzyme catalytic activities of a bacterial indole-3-acetyl-L-aspartic acid hydrolase [J].
Chou, JC ;
Welch, WH ;
Cohen, JD .
PLANT AND CELL PHYSIOLOGY, 2004, 45 (09) :1335-1341
[14]   The gene for indole-3-acetyl-L-aspartic acid hydrolase from Enterobacter agglomerans:: molecular cloning, nucleotide sequence, and expression in Escherichia coli [J].
Chou, JC ;
Mulbry, WW ;
Cohen, JD .
MOLECULAR AND GENERAL GENETICS, 1998, 259 (02) :172-178
[15]   CONVENIENT APPARATUS FOR THE GENERATION OF SMALL AMOUNTS OF DIAZOMETHANE [J].
COHEN, JD .
JOURNAL OF CHROMATOGRAPHY, 1984, 303 (01) :193-196
[16]   C-13(6)[BENZENE RING]-INDOLE-3-ACETIC ACID - A NEW INTERNAL STANDARD FOR QUANTITATIVE MASS-SPECTRAL ANALYSIS OF INDOLE-3-ACETIC-ACID IN PLANTS [J].
COHEN, JD ;
BALDI, BG ;
SLOVIN, JP .
PLANT PHYSIOLOGY, 1986, 80 (01) :14-19
[17]   CHEMISTRY AND PHYSIOLOGY OF THE BOUND AUXINS [J].
COHEN, JD ;
BANDURSKI, RS .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1982, 33 :403-430
[18]   Model legumes get the nod [J].
Cook, DR ;
VandenBosch, K ;
deBruijn, FJ ;
Huguet, T .
PLANT CELL, 1997, 9 (03) :275-281
[19]   The functional neuroanatomy of emotion and affective style [J].
Davidson, RJ ;
Irwin, W .
TRENDS IN COGNITIVE SCIENCES, 1999, 3 (01) :11-21
[20]   Expression studies on AUX1-like genes in Medicago truncatula suggest that auxin is required at two steps in early nodule development [J].
de Billy, F ;
Grosjean, C ;
May, S ;
Bennett, M ;
Cullimore, JV .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2001, 14 (03) :267-277