Single molecule force spectrometer with magnetic force control and inductive detection

被引：21

作者：

Schemmel, A ^{[1
]}

Gaub, HE ^{[1
]}

机构：

[1] Univ Munich, Lehrstuhl Angew Phys, D-80799 Munich, Germany

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 1999年 / 70卷 / 02期

关键词：

D O I：

10.1063/1.1149590

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Mechanical investigations with single molecules have opened a rich spectrum of new experiments, ranging from molecular recognition over polymer elasticity and DNA unzipping to protein unfolding. Here we present the design of a novel instrument, where the force is applied to the molecule via magnetic fields acting on a magnetic cantilever for atomic force microscopy. The performance of this instrument was demonstrated by measuring the binding force of biotin/avidin complexes. A new and technically very appealing detection scheme for measurements of unbinding forces was introduced, which consists of picking up the induced voltage pulse caused by a discontinuous change of the magnet position upon bond rupture. (C) 1999 American Institute of Physics. [S0034-6748(99)02602-7].

引用

页码：1313 / 1317

页数：5

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