DNA degradation at elevated temperatures after plasmid amplification in amino acid-starved Escherichia coli cells

被引：10

作者：

Neubauer, P

Wrobel, B

Wegrzyn, G

机构：

[1] UNIV GDANSK,DEPT BIOL MOLEC,GENET MOLEC LAB,PL-80822 GDANSK,POLAND

[2] UNIV HALLE WITTENBERG,INST BIOTECHNOL,D-06120 HALLE,GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 03期

关键词：

D O I：

10.1007/BF00142952

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Amino acid starvation of Escherichia coli relA mutants may be used as a method for efficient plasmid DNA amplification. Here we demonstrate. DNA degradation which occurs at elevated temperatures (42-43 degrees C) after plasmid amplification in amino acid-starved bacteria. These results may explain the, previously described low efficiency of plasmid DNA amplification at elevated temperatures.

引用

页码：321 / 326

页数：6

共 24 条

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BORRISS R, 1985, APPL MICROBIOL BIOT, V22, P63

[2]

CASHEL M, 1987, ESCHERICHIA COLI SAL, P1410

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