Selectivity tuning in an HPLC multicomponent separation

被引：12

作者：

Benická, E

Krupcík, J

Lehotay, J

Sandra, P

Armstrong, DW

机构：

[1] Slovak Univ Technol Bratislava, Dept Analyt Chem, FCHFT, Bratislava 81237, Slovakia

[2] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

[3] Iowa State Univ, Dept Chem, Ames, IA USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2005年 / 28卷 / 10期

关键词：

two columns coupled in series; selectivity tuning in HPLC;

D O I：

10.1081/JLC-200058326

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The optimisation of the selectivity of two columns coupled in series has been investigated in HPLC. Two columns with different polarities (PEG and CN) were coupled in series via a T connector and the retention of analytes chromatographed on the column system was tuned by varying the individual mobile phase flows in the coupled columns. The flow rate ratio necessary for receiving an optimum selectivity was calculated on the basis of the measured retention factors on the individual columns. The performance of this method has been studied by the separation of a model mixture of 17 analytes with various functionalities.

引用

页码：1453 / 1471

页数：19

共 19 条

[1]

BENICKA E, UNPUB

[2] Business-objective-directed, constraint-based multivariate optimization of high-performance liquid chromatography operational parameters [J].