Changes in pulsed-field gel electrophoresis patterns in clinical isolates of enterohemorrhagic Escherichia coli O157:H7 associated with loss of shiga toxin genes

被引：31

作者：

Murase, T

Yamai, S

Watanabe, H

机构：

[1] Kanagawa Prefectural Publ Hlth Lab, Dept Bacteriol & Pathol, Asahi Ku, Yokohama, Kanagawa 2410815, Japan

[2] Natl Inst Infect Dis, Dept Bacteriol, Tokyo 1628640, Japan

来源：

CURRENT MICROBIOLOGY | 1999年 / 38卷 / 01期

关键词：

D O I：

10.1007/PL00006771

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pulsed-field gel electrophoresis (PFGE) of XbaI-digested DNA fragments of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains showed disappearance of a 70- or 80-kb fragment in their patterns associated with loss of Shiga toxin genes during maintenance or subcultivation. Hybridization experiments with a DNA probe complementary to Shiga toxin sequences revealed that the Shiga toxin genes in the parental strain were located on fragments the same size as the lost fragments from the toxin-negative derivatives, The evidence indicates that PFGE pattern of EHEC O157:H7 may change due to loss of Shiga toxin genes, which is likely to be associated with curing of Shiga toxin gene carrying phages in vitro.

引用

页码：48 / 50

页数：3

共 19 条

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