High-throughput three-dimensional protein structure determination

被引:38
作者
Heinemann, U
Illing, G
Oschkinat, H
机构
[1] Max Delbruck Ctr Mol Med, Forsch Grp Kristallog, D-13125 Berlin, Germany
[2] Free Univ Berlin, Inst Chem Kristallog, D-14195 Berlin, Germany
[3] PSF Biotech AG, D-14059 Berlin, Germany
[4] Forschungsinst Mol Pharmakol, D-13125 Berlin, Germany
关键词
D O I
10.1016/S0958-1669(00)00226-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the wake of finished genomic sequencing projects, high-throughput analysis techniques are being developed in various fields of functional genomics. Of special interest in this regard is the three-dimensional structure analysis of proteins by X-ray crystallography and NMR spectroscopy, which has been characterized by distinctly low-throughput in the past. A number of recent advances in instrumentation and software are promising to radically change this situation, leaving the production of suitable protein samples as the sole rate-limiting step in structural analyses.
引用
收藏
页码:348 / 354
页数:7
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