Mapping the surface characteristics of polystyrene microtiter wells by a multimode scanning force microscopy approach

被引:7
作者
Allen, S
Connell, SDA
Chen, X
Davies, J
Davies, MC
Dawkes, AC [1 ]
Roberts, CJ
Tendler, SJB
Williams, PM
机构
[1] Univ Nottingham, Lab Biophys & Surface Anal, Sch Pharmaceut Sci, Nottingham NG7 2RD, England
[2] OrthoClin Diagnost, Amersham HP7 0HJ, Bucks, England
基金
英国生物技术与生命科学研究理事会;
关键词
scanning force microscopy; spatial mapping; immunoassay; microtiter well surfaces;
D O I
10.1006/jcis.2001.7800
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Polystyrene microtiter wells are commonly employed as substrates for immunoassays and for molecular screening. The full characterization of such substrates is therefore critical for their optimal use in such settings. In this study, various scanning force microscopy analysis modes are employed for their surface characterization. This is achieved through the spatial mapping of probe-sample interactions via lateral force microscopy, force-curve mapping, phase imaging, and pulsed force microscopy. Previous surface analytical studies had indicated the presence of a volatile hydrocarbon contaminant on well surfaces, which resulted in areas resistant to protein adsorption. These studies, however, were unable to directly determine the contaminant's dimensions or its distribution over the substrate. The obtained results confirm the presence of discrete patches of a hydrophobic material on the well surface, which additionally are found to behave as a viscous fluid. Such findings concur with the predictions made in the previous study and illustrate the potential benefits of a combined surface analytical approach. (C) 2001 Academic Press.
引用
收藏
页码:470 / 476
页数:7
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