Rapid and accurate determination of genotypic clarithromycin resistance in cultured Helicobacter pylori by fluorescent in situ hybridization

被引:49
作者
Rüssmann, H
Adler, K
Haas, R
Gebert, B
Koletzko, S
Heesemann, J
机构
[1] Univ Munich, Max von Pettenkofer Inst Hyg & Med Mikrobiol, D-80336 Munich, Germany
[2] Univ Munich, Dr v Haunersches Kinderspital, D-80336 Munich, Germany
关键词
D O I
10.1128/JCM.39.11.4142-4144.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Phenotypic susceptibility testing for clarithromycin by E-test and disk diffusion of 109 cultured Helicobacter pylori isolates was compared with the genotypic susceptibility determination by fluorescent in situ hybridization (FISH). No discrepancies were found between these three methods. However, FISH has the advantage of providing results after 3 h.
引用
收藏
页码:4142 / 4144
页数:3
相关论文
共 14 条
[1]   Antibiotic resistance in Helicobacter pylori:: Implications for therapy [J].
Graham, DY .
GASTROENTEROLOGY, 1998, 115 (05) :1272-1277
[2]  
Maeda S, 2000, J CLIN MICROBIOL, V38, P210
[3]   Helicobacter pylori specific nested PCR assay for the detection of 23S rRNA mutation associated with clarithromycin resistance [J].
Maeda, S ;
Yoshida, H ;
Ogura, K ;
Kanai, F ;
Shiratori, Y ;
Omata, M .
GUT, 1998, 43 (03) :317-321
[4]  
Malfertheiner P, 1997, GUT, V41, P8
[5]   Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium [J].
Matsumura, M ;
Hikiba, Y ;
Ogura, K ;
Togo, G ;
Tsukuda, I ;
Ushikawa, K ;
Shiratori, Y ;
Omata, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (02) :691-695
[6]  
Mégraud F, 1998, BRIT MED BULL, V54, P207
[7]   Macrolide resistance in Helicobacter pylori: Rapid detection of point mutations and assays of macrolide binding to ribosomes [J].
Occhialini, A ;
Urdaci, M ;
DoucetPopulaire, F ;
Bebear, CM ;
Lamouliatte, H ;
Megraud, F .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (12) :2724-2728
[8]   Detection of point mutations associated with resistance of Helicobacter pylori to clarithromycin by hybridization in liquid phase [J].
Pina, M ;
Occhialini, A ;
Monteiro, L ;
Doermann, HP ;
Mégraud, F .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) :3285-3290
[9]   Comparison of fluorescent in situ hybridization and conventional culturing for detection of Helicobacter pylori in gastric biopsy specimens [J].
Rüssmann, H ;
Kempf, VAJ ;
Koletzko, S ;
Heesemann, J ;
Autenrieth, IB .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :304-308
[10]   A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori [J].
Stone, GG ;
Shortridge, D ;
Versalovic, J ;
Beyer, J ;
Flamm, RK ;
Graham, DY ;
Ghoneim, AT ;
Tanaka, SK .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (03) :712-714