A recirculating-flow fluorescent oxygen sensor

被引：5

作者：

Towe, BC ^{[1
]}

Flechsig, S ^{[1
]}

Spaulding, G ^{[1
]}

机构：

[1] NASA,JOHNSON SPACE CTR,HOUSTON,TX 77059

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 08期

关键词：

oxygen sensor; fluorescent biosensor;

D O I：

10.1016/0956-5663(96)85931-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An immersible oxygen sensor was constructed by circulating small quantities of ruthenium tris-(2,2'-bipyridyl) II dichloride oxygen-sensitive fluorescent dye through a loop of oxygen-permeable silicone tubing immersed in test medium. The fluroescence intensity of the dye was subsequently measured as it exited the flow loop and related to oxygen tension. This method of measuring the oxygen tension, through diffusive transport to a flowing stream of dye and recirculating it in the sensor, has been found to give a stable response and relatively long sensor lifetime without major recalibration. The sensor showed good stability over at least a week's duration and showed no degradation due to leaching of the dye through membranes or photobleaching that commonly affects fluorescent sensors with immobilized chemistries. (C) 1996 Elsevier Science Limited

引用

页码：799 / 803

页数：5

共 11 条

[1] OPTICAL FLUORESCENCE AND ITS APPLICATION TO AN INTRAVASCULAR BLOOD-GAS MONITORING-SYSTEM [J].