Free-standing protein films for dynamic mode detection of cations binding

被引:1
作者
Saya, D
Coleman, AW
Lazar, AN
Bergaud, C
Nicu, L [1 ]
机构
[1] CNRS, Lab Anal & Architecture Syst, F-31077 Toulouse, France
[2] CNRS, Inst Biol & Chem Prot, F-69367 Lyon, France
[3] Univ Tokyo, Lab Integrated Micro Mechatron Syst, LIMMS CNRS IIS UMi2820, Inst Ind Sci, Tokyo 1538505, Japan
关键词
D O I
10.1063/1.2035872
中图分类号
O59 [应用物理学];
学科分类号
摘要
This letter reports on the investigation of the mechano-chemical effect of cross-linked dried free-standing alpha-lactalbumin (alpha-lactalbumin) thin films induced by different cation, calcium, magnesium, and potassium binding. The protein membranes were fabricated by drying droplets of an alpha-lactalbumin solution on top of silicon through-wafer holes obtained by deep reactive ion etching. Then the membranes were consecutively exposed to solutions of the cations in HEPES buffer solution while their resonant frequencies were measured by full-field surface stroboscopic white light interferometry. Tests on more than 30 free-standing protein films showed more significant conformational changes of the alpha-lactalbumin after immersion in a calcium solution than those observed after immersion in magnesium and potassium solutions. These results demonstrate, the real potential of free-standing protein films to be used as resonant biosensors for multiple cation detection. (c) 2005 American Institute of Physics.
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