Fabrication of protein gradients for cell culture using a miniature squeegee

被引：2

作者：

Costantino, Santiago ^{[3
]}

McQuinn, Christopher G. ^{[1
]}

Kennedy, Timothy E. ^{[2
]}

Wiseman, Paul W. ^{[1
,3
]}

机构：

[1] McGill Univ, Dept Chem, McGill Program NeuroEngn, Montreal, PQ, Canada

[2] McGill Univ, Dept Neurol & Neurosurg, McGill Program NeuroEngn, Montreal, PQ, Canada

[3] McGill Univ, Dept Phys, McGill Program NeuroEngn, Montreal, PQ, Canada

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2008年 / 70卷 / 06期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

protein gradient; protein patterning; axon guidance; substrate modification;

D O I：

10.1016/j.jbbm.2007.05.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We present a straightforward method to create spatial gradients of substrate bound protein for live cell studies using only mechanical parts. Protein concentration gradients on a micron scale can be fabricated in several minutes for a relatively low cost using a method that is generally applicable to any protein and substrate combination. We describe the details of the device construction, and provide examples of mammalian cells grown on substrates patterned with protein concentration gradients using this technique. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1192 / 1195

页数：4

共 16 条

[1]

Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide [J].