The use of 7-amino-actinomycin D in the analysis of Candida albicans phagocytosis and opsonization

被引：12

作者：

Ortega, E ^{[1
]}

Algarra, I ^{[1
]}

Serrano, MJ ^{[1
]}

de Cienfuegos, GA ^{[1
]}

Gaforio, JJ ^{[1
]}

机构：

[1] Univ Jaen, Fac Expt Sci, Dept Hlth Sci, Microbiol Unit, Jaen 23071, Spain

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2001年 / 253卷 / 1-2期

关键词：

7-amino-actinomycin D; phagocytosis; opsonization; Candida albicans; flow cytometry; macrophages;

D O I：

10.1016/S0022-1759(01)00358-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the use of 7-amino-actinomycin D (7AAD) to measure phagocytosis and the opsonizing capacity of serum. Heat-inactivated Candida albicans was previously stained with 7AAD and incubated with resident peritoneal macrophages. The samples were analyzed by flow cytometry and phagocytic cells were identified by their bright red fluorescence. This is a rapid, reproducible and reliable one-step procedure and provides a means of evaluating low levels of phagocytosis. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：189 / 193

页数：5

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