Detection of substance P and its receptor in human fetal microglia

被引：59

作者：

Lai, JP ^{[1
]}

Zhan, GX ^{[1
]}

Campbell, DE ^{[1
]}

Douglas, SD ^{[1
]}

Ho, WZ ^{[1
]}

机构：

[1] Univ Penn, Childrens Hosp Philadelphia, Joseph Stokes Jr Res Inst, Sch Med,Dept Pediat,Div Immunol & Infect Dis, Philadelphia, PA 19104 USA

来源：

NEUROSCIENCE | 2000年 / 101卷 / 04期

关键词：

preprotachykinin-A; neurokinin-1; receptor; RT-PCR; macrophages; monocytes;

D O I：

10.1016/S0306-4522(00)00398-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Substance P, the most abundant neurokinin in the CNS, is a major modulator of the immune system. We have examined the gene expression of substance P and its receptor in human fetal brain microglia. Using reverse transcription-polymerase chain reaction and Southern blotting assay, the four isoforms of preprotachykinin-A gene transcripts (alpha, beta, gamma and delta) were detected in the microglia. The human fetal microglia produced significantly higher levels of endogenous substance P protein (640-850 pg/10(6) cells) than did human peripheral blood monocyte-derived macrophages (25-50pg/10(6) cells), as determined by an enzyme immunoassay. Using immunohistochemical staining with an anti-substance P antibody, cell membrane substance P immunoreactivity was observed. In addition, we identified the presence of messenger RNA for neurokinin-1 receptor, a primary receptor for substance P in human fetal microglia. From these data, we propose that substance P and its receptor are biologically involved in regulating the functions of microglia, and potentially play an important role in host defense of the central nervous system. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1137 / 1144

页数：8

共 40 条

[1] SELECTIVE LOCALIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE AND SUBSTANCE-P IN HUMAN EOSINOPHILS [J].