Protective CD4+ and CD8+ T cells against influenza virus induced by vaccination with nucleoprotein DNA

被引：190

作者：

Ulmer, JB ^{[1
]}

Fu, TM ^{[1
]}

Deck, RR ^{[1
]}

Friedman, A ^{[1
]}

Guan, LM ^{[1
]}

DeWitt, C ^{[1
]}

Liu, X ^{[1
]}

Wang, S ^{[1
]}

Liu, MA ^{[1
]}

Donnelly, JJ ^{[1
]}

Caulfield, MJ ^{[1
]}

机构：

[1] Merck Res Labs, Dept Virus & Cell Biol, W Point, PA 19486 USA

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 07期

关键词：

D O I：

10.1128/JVI.72.7.5648-5653.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA vaccination is an effective means of eliciting both humoral and cellular immunity, including cytotoxic T lymphocytes (CTL). Using an influenza virus model, we previously demonstrated that injection of DNA encoding influenza virus nucleoprotein (NP) induced major histocompatibility complex class I-restricted CTL and cross strain protection from lethal virus challenge in mice (J, B, Ulmer et al., Science 259:1745-1749, 1993), In the present study, we have characterized in more detail the cellular immune responses induced by NP DNA, which included robust lymphoproliferation and Th1-type cytokine secretion (high levels of gamma interferon and interleukin-2 [IL-2], with little IL-4 or IL-10) in response to antigen specific restimulation of splenocytes in vitro. These responses were mediated by CD4(+) T cells, as shown by in vitro depletion of T-cell subsets. Taken together, these results indicate that immunization with NP DNA primes both cytolytic CD8(+) T cells and cytokine-secreting CD4(+) T cells. Further, we demonstrate by adoptive transfer and in vivo depletion of T-cell subsets that both of these types of T cells act as effecters in protective immunity against influenza virus challenge conferred by NP DNA.

引用

页码：5648 / 5653

页数：6

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