Expression cloning of Xantivin, a Xenopus lefty/antivin-related gene, involved in the regulation of activin signaling during mesoderm induction

被引:49
作者
Tanegashima, K
Yokota, C
Takahashi, S
Asashima, M
机构
[1] Univ Tokyo, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
[2] Univ Tokyo, CREST Project, Meguro Ku, Tokyo 1538902, Japan
关键词
mesoderm induction; activin signaling; nodal-related genes; lefty; antivin; Xenopus laevis; feedback inhibitor;
D O I
10.1016/S0925-4773(00)00465-2
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In a screening for activin-responsive genes, we isolated a Xenopus lefty/antivin-related gene, called Xantivin (Xatv). In the animal cap assay, the expression of Xatv was induced by activin signaling, and in the embryo, by nodal-related genes. Overexpression of Xatv in the marginal zone caused suppression of mesoderm formation and gastrulation defects, and inhibited the secondary axis formation induced by Xnr1 and Xactivin, suggesting that Xatv acted as a feedback inhibitor of activin signaling. However, in the animal cap, Xatv failed to antagonize Xnr1 and Xactivin. This result suggested that Xatv has different responses in the marginal zone and in the animal region, and antagonizes to a higher degree activin signaling in the marginal zone. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:3 / 14
页数:12
相关论文
共 73 条
[51]   Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling [J].
Sampath, K ;
Rubinstein, AL ;
Cheng, AHS ;
Liang, JO ;
Fekany, K ;
Solnica-Krezel, L ;
Korzh, V ;
Halpern, ME ;
Wright, CVE .
NATURE, 1998, 395 (6698) :185-189
[52]   XENOPUS CHORDIN - A NOVEL DORSALIZING FACTOR-ACTIVATED BY ORGANIZER-SPECIFIC HOMEOBOX GENES [J].
SASAI, Y ;
LU, B ;
STEINBEISSER, H ;
GEISSERT, D ;
GONT, LK ;
DEROBERTIS, EM .
CELL, 1994, 79 (05) :779-790
[53]   Nodal signalling in vertebrate development [J].
Schier, AF ;
Shen, MM .
NATURE, 2000, 403 (6768) :385-389
[54]   A SIMPLE SUBTRACTIVE HYBRIDIZATION TECHNIQUE EMPLOYING PHOTOACTIVATABLE BIOTIN AND PHENOL EXTRACTION [J].
SIVE, HL ;
STJOHN, T .
NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10937-10937
[55]   EXPRESSION OF A XENOPUS HOMOLOG OF BRACHYURY (T) IS AN IMMEDIATE-EARLY RESPONSE TO MESODERM INDUCTION [J].
SMITH, JC ;
PRICE, BMJ ;
GREEN, JBA ;
WEIGEL, D ;
HERRMANN, BG .
CELL, 1991, 67 (01) :79-87
[56]   EXPRESSION CLONING OF NOGGIN, A NEW DORSALIZING FACTOR LOCALIZED TO THE SPEMANN ORGANIZER IN XENOPUS EMBRYOS [J].
SMITH, WC ;
HARLAND, RM .
CELL, 1992, 70 (05) :829-840
[57]   INJECTED XWNT-8 RNA ACTS EARLY IN XENOPUS EMBRYOS TO PROMOTE FORMATION OF A VEGETAL DORSALIZING CENTER [J].
SMITH, WC ;
HARLAND, RM .
CELL, 1991, 67 (04) :753-765
[58]   INJECTED WNT RNA INDUCES A COMPLETE BODY AXIS IN XENOPUS EMBRYOS [J].
SOKOL, S ;
CHRISTIAN, JL ;
MOON, RT ;
MELTON, DA .
CELL, 1991, 67 (04) :741-752
[59]   Differential expression of VegT and Antipodean protein isoforms in Xenopus [J].
Stennard, F ;
Zorn, AM ;
Ryan, K ;
Garrett, N ;
Gurdon, JB .
MECHANISMS OF DEVELOPMENT, 1999, 86 (1-2) :87-98
[60]  
Stennard F, 1996, DEVELOPMENT, V122, P4179