Solid-State Protein-Structure Determination with Proton-Detected Triple-Resonance 3D Magic-Angle-Spinning NMR Spectroscopy

被引：178

作者：

Zhou, Donghua H.

Shea, John J.

Nieuwkoop, Andrew J.

Franks, W. Trent

Wylie, Benjamin J.

Mullen, Charles

Sandoz, Dennis

Rienstra, Chad M.

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Varian Associates Inc, Ft Collins, CO USA

[3] Varian Associates Inc, Palo Alto, CA 94303 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 44期

关键词：

indirect proton detection; magic-angle spinning; NMR spectroscopy; protein structures;

D O I：

10.1002/anie.200702905

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. (Graph Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：8380 / 8383

页数：4

相关论文

共 30 条

[1] CHEMICAL-SHIFT CORRELATION SPECTROSCOPY IN ROTATING SOLIDS - RADIO FREQUENCY-DRIVEN DIPOLAR RECOUPLING AND LONGITUDINAL EXCHANGE [J].

BENNETT, AE ;

OK, JH ;

GRIFFIN, RG ;

VEGA, S .

JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (11) :8624-8627

[2] NATURAL ABUNDANCE N-15 NMR BY ENHANCED HETERONUCLEAR SPECTROSCOPY [J].

BODENHAUSEN, G ;

RUBEN, DJ .

CHEMICAL PHYSICS LETTERS, 1980, 69 (01) :185-189

[3] Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy [J].

Castellani, F ;

van Rossum, B ;

Diehl, A ;

Schubert, M ;

Rehbein, K ;

Oschkinat, H .

NATURE, 2002, 420 (6911) :98-102

[4] Ultrahigh resolution in proton solid-state NMR spectroscopy at high levels of deuteration [J].

Chevelkov, V ;

Rehbein, K ;

Diehl, A ;

Reif, B .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (23) :3878-3881

[5] 1H detection in MAS solid-state NMR Spectroscopy of biomacromolecules employing pulsed field gradients for residual solvent suppression [J].

Chevelkov, V ;

van Rossum, BJ ;

Castellani, F ;

Rehbein, K ;

Diehl, A ;

Hohwy, M ;

Steuernagel, S ;

Engelke, F ;

Oschkinat, H ;

Reif, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (26) :7788-7789

[6]

Chevelkov V., 2006, ANGEW CHEM, V118, P3963

[7] Protein backbone angle restraints from searching a database for chemical shift and sequence homology [J].

Cornilescu, G ;

Delaglio, F ;

Bax, A .

JOURNAL OF BIOMOLECULAR NMR, 1999, 13 (03) :289-302

[8] NMRPIPE - A MULTIDIMENSIONAL SPECTRAL PROCESSING SYSTEM BASED ON UNIX PIPES [J].

DELAGLIO, F ;

GRZESIEK, S ;

VUISTER, GW ;

ZHU, G ;

PFEIFER, J ;

BAX, A .

JOURNAL OF BIOMOLECULAR NMR, 1995, 6 (03) :277-293

[9] Solid-state nuclear magnetic resonance spectra of dipolar-coupled multi-spin systems under fast magic angle spinning [J].

Filip, C ;

Hafner, S ;

Schnell, I ;

Demco, DE ;

Spiess, HW .

JOURNAL OF CHEMICAL PHYSICS, 1999, 110 (01) :423-440

[10] SOLARIA: A protocol for automated cross-peak assignment and structure calculation for solid-state magic-angle spinning NMR spectroscopy [J].

Fossi, M ;

Castellani, T ;

Nilges, M ;

Oschkinat, H ;

van Rossum, BJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (38) :6151-6154