The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: Phototoxic influence on the DNA

被引：4

作者：

Yashchuk, Valeriy M. ^{[1
,2
]}

Kudrya, Vladyslav Yu. ^{[1
,2
,3
]}

Losytskyy, Mykhaylo Yu. ^{[1
,2
,4
]}

Tokar, Valentyna P. ^{[1
,2
,4
]}

Yarmoluk, Sergiy M. ^{[4
]}

Dmytruk, Igor M. ^{[1
,2
]}

Prokopets, Vadym M. ^{[1
,2
]}

Kovalska, Vladyslava B. ^{[4
]}

Balanda, Anatoliy O. ^{[4
]}

Kryvorotenko, Dmytro V. ^{[4
]}

Ogul'chansky, Tymish Yu. ^{[5
,6
]}

机构：

[1] Kyiv Taras Shevchenko Natl Univ, Phys Fac, Build 1,Acad Glushkov Ave 2, UA-03680 Kiev, Ukraine

[2] Kyiv Taras Shevchenko Natl Univ, Chem Fac, UA-03680 Kiev, Ukraine

[3] Natl Agr Univ Ukraine, Kiev, Ukraine

[4] NASU, Inst Mol Biol & Genet, Kiev, Ukraine

[5] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA

[6] SUNY Buffalo, Inst Lasers Photon & Biophoton, Buffalo, NY 14260 USA

来源：

PHOTONICS NORTH 2007, PTS 1 AND 2 | 2007年 / 6796卷

关键词：

DNA detection; fluorescent probes; two-photon excitation; phototoxicity; photostability;

D O I：

10.1117/12.778096

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The optical absorption, fluorescence and phosphorescence of the novel styryl dyes developed for the fluorescent detection of DNA were investigated. The energy structures of dye molecules as well as spectral manifestations of the dyes aggregate formation and interaction with DNA were studied. The dramatic increase (up to 1000 times) of the fluorescence intensity of dyes in the presence of DNA was observed. The photostability and phototoxic influence on the DNA of several styryl dyes were studied by analyzing absorption, fluorescence and phosphorescence spectra of these dyes and dye-DNA systems. Changes of the optical density value of 4 dye-DNA solutions caused by the visible light irradiation were fixed in the wavelength regions of the DNA absorption and of the dye absorption. Fluorescence emission of dye-DNA complexes upon two-photon excitation (TPE) at wavelength 1064 nm with the 20 ns pulsed YAG:Nd3+ laser and at 840 nm with the 90 fs pulsed Ti:sapphire laser was registered. The values of two-photon absorption cross-sections of dye-DNA complexes were evaluated.

引用

页数：14

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