Brucella control of dendritic cell maturation is dependent on the TIR-Containing protein btp1

被引：233

作者：

Salcedo, Suzana P. ^{[1
,2
,3
]}

Marchesini, Maria Ines ^{[4
]}

Lelouard, Hugues ^{[1
,2
,3
]}

Fugier, Emilie ^{[1
,2
,3
]}

Jolly, Gilles ^{[1
,2
,3
]}

Balor, Stephanie ^{[1
,2
,3
]}

Muller, Alexandre ^{[1
,2
,3
]}

Lapaque, Nicolas ^{[1
,2
,3
]}

Demaria, Olivier ^{[1
,2
,3
]}

Alexopoulou, Lena ^{[1
,2
,3
]}

Comerci, Diego J. ^{[4
,5
]}

Ugalde, Rodolfo A. ^{[4
,5
]}

Pierre, Philippe ^{[1
,2
,3
]}

Gorvel, Jean-Pierre ^{[1
,2
,3
]}

机构：

[1] Aix Marseille Univ, Fac Sci Luminy, Ctr Immunol Marseille Luminy, Marseille, France

[2] INSERM, U631, Marseille, France

[3] UMR 6102, CNRS, Marseille, France

[4] Univ Nacl San Martin, IIB INTECH, Inst Invest Biotecnol, Buenos Aires, DF, Argentina

[5] CONICET INTI Ed 24, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina

来源：

PLOS PATHOGENS | 2008年 / 4卷 / 02期

关键词：

D O I：

10.1371/journal.ppat.0040021

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Brucella is an intracellular pathogen able to persist for long periods of time within the host and establish a chronic disease. We show that soon after Brucella inoculation in intestinal loops, dendritic cells from ileal Peyer's patches become infected and constitute a cell target for this pathogen. In vitro, we found that Brucella replicates within dendritic cells and hinders their functional activation. In addition, we identified a new Brucella protein Btp1, which down-modulates maturation of infected dendritic cells by interfering with the TLR2 signaling pathway. These results show that intracellular Brucella is able to control dendritic cell function, which may have important consequences in the development of chronic brucellosis.

引用

页数：16

共 52 条

[1] BOVINE ILEAL DOME LYMPHOEPITHELIAL CELLS - ENDOCYTOSIS AND TRANSPORT OF BRUCELLA-ABORTUS STRAIN-19 [J].

ACKERMANN, MR ;

CHEVILLE, NF ;

DEYOE, BL .

VETERINARY PATHOLOGY, 1988, 25 (01) :28-35

[2] Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function [J].

Adachi, O ;

Kawai, T ;

Takeda, K ;

Matsumoto, M ;

Tsutsui, H ;

Sakagami, M ;

Nakanishi, K ;

Akira, S .

IMMUNITY, 1998, 9 (01) :143-150

[3] Cyclic β-1,2-glucan is a brucella virulence factor required for intracellular survival [J].

Arellano-Reynoso, B ;

Lapaque, N ;

Salcedo, S ;

Briones, G ;

Ciocchini, AE ;

Ugalde, R ;

Moreno, E ;

Moriyón, I ;

Gorvel, JP .

NATURE IMMUNOLOGY, 2005, 6 (06) :618-625

[4] A polymeric bacterial protein activates dendritic cells via TLR4 [J].

Bergner, PM ;

Mundiñano, J ;

Piazzon, I ;

Goldbaum, FA .

JOURNAL OF IMMUNOLOGY, 2006, 176 (04) :2366-2372

[5] High susceptibility of human dendritic cells to invasion by the intracellular pathogens Brucella suis, Brucella abortus, and Brucella melitensis [J].

Billard, E ;

Cazevieille, C ;

Dornand, J ;

Gross, A .

INFECTION AND IMMUNITY, 2005, 73 (12) :8418-8424

[6] Brucella suis prevents human dendritic cell maturation and antigen presentation through regulation of tumor necrosis factor alpha secretion [J].

Billard, Elisabeth ;

Dornand, Jacques ;

Gross, Antoine .

INFECTION AND IMMUNITY, 2007, 75 (10) :4980-4989

[7] Francisella tularensis induces aberrant activation of pulmonary dendritic cells [J].

Bosio, CM ;

Dow, SW .

JOURNAL OF IMMUNOLOGY, 2005, 175 (10) :6792-6801

[8] Brucella abortus cyclic β-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in HeLa cells [J].

Briones, G ;

De Iannino, NI ;

Roset, M ;

Vigliocco, A ;

Paul, PS ;

Ugalde, RA .

INFECTION AND IMMUNITY, 2001, 69 (07) :4528-4535

[9] Brucella coopts the small GTPase Sar1 for intracellular replication [J].

Celli, J ;

Salcedo, SP ;

Gorvel, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (05) :1673-1678

[10] Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum [J].

Celli, J ;

de Chastellier, C ;

Franchini, DM ;

Pizarro-Cerda, J ;

Moreno, E ;

Gorvel, AP .

JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (04) :545-556

← 1 2 3 4 5 6 →