Novel nuclear intron-spanning primers for Arecaceae evolutionary biology

被引：18

作者：

Bacon, Christine D. ^{[1
]}

Feltus, F. Alex ^{[2
]}

Paterson, Andrew H. ^{[2
]}

Bailey, C. Donovan ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA

[2] Univ Georgia, Plant Genome Mapping Lab, Athens, GA 30602 USA

来源：

MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 01期

关键词：

Arecaceae; CISP; conserved intron-scanning primers; intron; nuclear gene; Palmae;

D O I：

10.1111/j.1471-8286.2007.01928.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, 96 nuclear 'conserved intron-scanning primers' were screened across subfamilies the Arecaceae (palms) for potential use in research focused on palm evolutionary biology. Primers were evaluated based on their ability to amplify single polymerase chain reaction products in Arecaceae, the clarity of sequencing reads, and the interspecific variability observed. Ultimately, the results suggest that: (i) seven of the loci are likely to be suitable when comparing non-Arecaceae outgroups and Arecaceae ingroups; (ii) seven loci may be of use when comparing subfamilies of Arecaceae; and (iii) four of the loci may be of use when comparing closely related genera.

引用

页码：211 / 214

页数：4