Influence of Temperature during Transportation on Cell-Free DNA Analysis

被引：67

作者：

Hidestrand, Mats ^{[1
]}

Stokowski, Renee ^{[4
]}

Song, Ken ^{[4
]}

Oliphant, Arnold ^{[4
]}

Deavers, Julie ^{[1
]}

Goetsch, Mary ^{[1
]}

Simpson, Pippa ^{[2
]}

Kuhlman, Randall ^{[3
]}

Ames, Mary ^{[5
]}

Mitchell, Michael ^{[1
]}

Tomita-Mitchell, Aoy ^{[1
]}

机构：

[1] Med Coll Wisconsin, Dept Surg, Div Cardiothorac Surg, Milwaukee, WI 53226 USA

[2] Med Coll Wisconsin, Dept Pediat, Milwaukee, WI 53226 USA

[3] Med Coll Wisconsin, Dept Obstet & Gynecol, Milwaukee, WI 53226 USA

[4] Aria Diagnost Inc, San Jose, CA USA

[5] Oregon Hlth & Sci Univ, Dept Gynecol, Div Maternal Fetal Med, Portland, OR 97201 USA

来源：

FETAL DIAGNOSIS AND THERAPY | 2012年 / 31卷 / 02期

关键词：

Streck blood collection tubes; Shipping whole blood; Cell-free DNA; BLOOD-PROCESSING PROTOCOLS; FREE FETAL DNA; MATERNAL PLASMA; QUANTIFICATION; ANEUPLOIDY; DIAGNOSIS; SERUM;

D O I：

10.1159/000335020

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To determine the effect of shipping blood in Streck blood collection tubes (BCT) prior to processing on cell-free DNA (cf-DNA) levels. Methods: Blood was collected in ethylenediaminetetraacetic acid (EDTA) and BCT tubes from 10 pregnant women carrying male fetuses. One set of each tube for each subject was processed to plasma immediately (standard cf-DNA protocol), whereas the other set was shipped by air courier and then processed. DNA was extracted and total and fetal DNA concentrations were measured by TaqMan multiplexed quantitative real-time PCR. Results: No significant difference was observed in total cf-DNA in plasma between immediately processed EDTA (control) and immediately processed BCT samples. Moreover, no significant change in total cf-DNA was detected in plasma of BCT samples shipped at room temperature. Significant differences in total cf-DNA leading to a significantly decreased fetal fraction were found in shipped EDTA samples and BCT samples shipped at 4 degrees C. Discussion: BCT tubes are suitable for shipping whole blood prior to processing with respect to cf-DNA levels. However, care should be taken to ensure that samples are not exposed to extreme temperatures during shipment. This finding will benefit the development and clinical application of noninvasive methods of fetal diagnosis that utilize cf-DNA. Copyright (C) 2012 S. Karger AG, Basel

引用

页码：122 / 128

页数：7

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