Identification of specific PP2A complexes involved in human cell transformation

被引：295

作者：

Chen, W

Possemato, R

Campbell, KT

Plattner, CA

Pallas, DC

Hahn, WC

机构：

[1] Brigham & Womens Hosp, Dept Med, Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[4] Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA

[5] Emory Univ, Sch Med, Winship Canc Inst, Atlanta, GA 30322 USA

来源：

CANCER CELL | 2004年 / 5卷 / 02期

关键词：

D O I：

10.1016/S1535-6108(04)00026-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The SV40 small t antigen (ST) interacts with the serine-threonine protein phosphatase 2A (PP2A). To investigate the role of this interaction in transformation, we suppressed the expression of the PP2A B56gamma subunit in human embryonic kidney (HEK) epithelial cells expressing SV40 large T antigen, hTERT, and H-RAS. Suppression of PP2A B56gamma expression inhibited PP2A-specific phosphatase activity similar to that achieved by ST and conferred the ability to grow in an anchorage-independent fashion and to form tumors. Overexpression of PP2A B56gamma3 in tumorigenic HEK cells expressing ST or human lung cancer cell lines partially reversed the tumorigenicity of these cells. These observations identify specific PP2A complexes involved in human cell transformation.

引用

页码：127 / 136

页数：10

共 56 条

[11] A developmentally regulated, neuron-specific splice variant of the variable subunit Bβ targets protein phosphatase 2A to mitochondria and modulates apoptosis [J].