Unsaturated diether lipids in the psychrotrophic archaeon Halorubrum lacusprofundi

被引:41
作者
Gibson, JAE
Miller, MR
Davies, NW
Neill, GP
Nichols, DS
Volkman, JK
机构
[1] Univ Tasmania, IASOS, Hobart, Tas 7001, Australia
[2] CSIRO, Marine Res, Hobart, Tas 7001, Australia
[3] Univ Tasmania, Sch Agr Sci, Hobart, Tas 7001, Australia
[4] Univ Tasmania, Cent Sci Lab, Hobart, Tas 7001, Australia
[5] Univ Tasmania, Dept Chem, Hobart, Tas 7001, Australia
关键词
archaea; unsaturated ether lipids; halophile; temperature-dependence; antarctica; psychrotroph; APCI; electrospray;
D O I
10.1016/j.syapm.2004.09.004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The major phospholipids of Halorubrum lacusprofundi grown at 25 degreesC were archaeol phosphatidylglycerol, archaeol phosphatidylglycerylsulphate and archaeol phosphatidylglycerylphosphate methyl ester. Glycolipids included a monoglycosyl archaeol and the sulphate ester of a diglycosyl archaeol. Cultures grown at 12degreesC contained the same suite of phospho- and glycolipids, with the addition of a series of unsaturated analogues with up to six double bonds. The patterns of unsaturation were similar for all the phospholipid series, but a different pattern occurred in the glycolipids. The analytical techniques used in this study allow facile detection of unsaturated archaeal cell membrane lipids that are degraded by commonly used chemical derivatization procedures. (C) 2004 Elsevier GmbH. All rights reserved.
引用
收藏
页码:19 / 26
页数:8
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