Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene

被引：3

作者：

Dharmsthiti, S ^{[1
]}

Luchai, S ^{[1
]}

机构：

[1] Mahidol Univ, Ctr Biotechnol, Inst Res & Dev Sci & Technol, Nakhon Pathom 73170, Pathom, Thailand

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1998年 / 86卷 / 03期

关键词：

heterologous lipase; Acinetobacter calcoaceticus; Aeromonas sobria; immobilization;

D O I：

10.1016/S0922-338X(98)80140-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aeromonas sobria LP004 harboring the Acinetobacter calcoaceticus lipase gene was designated as strain LP094. Lipase production of LP094 in WYGS medium [Lotrakul and Dharmsthiti, World J. Microbiol. Biotechnol., 13:163-166, 1997] was scaled up to a 50-l volume. LP094 lipase immobilized by ionic binding to either IR120 (Na+) or IRC50 (H+) Amberlite resins was found to retain more than 90% activity after 15 d storage at room temperature (approximate to 25 to 30 degrees C) or at 4 degrees C. After 5 repeated lipid hydrolysis reactions, the activities of both immobilized preparations remained higher than 65%.

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页码：335 / 337

页数：3

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