Molecular characterization of mtDNA depleted and repleted NT2 cell lines

被引：20

作者：

Binder, DR ^{[1
]}

Dunn, WH ^{[1
]}

Swerdlow, RH ^{[1
]}

机构：

[1] Univ Virginia, Hlth Syst, Dept Neurol, Charlottesville, VA 22908 USA

来源：

MITOCHONDRION | 2005年 / 5卷 / 04期

关键词：

mitochondrial DNA; NT2; cells; transmitochondrial cybrids;

D O I：

10.1016/j.mito.2005.04.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transmitochondrial cytoplasmic hybrids (cybrids) enable functional assessment of mitochondrial DNA (mtDNA)-encoded proteins. Cybrid production often utilizes cell lines depleted of endogenous mtDNA (p0 cells), and a number of suitable rho 0 cell lines exist for this purpose. We now provide molecular data characterizing an NT2 human teratocarcinoma rho 0 cell line, as well as NT2 cybrid derivatives. NT2 rho 0 cells contained no detectable mtDNA on a sensitive PCR assay. Eight weeks after exogenous mtDNA transfer cybrids showed no evidence of endogenous mtDNA reversion, and heteroplasmic ratios of a single nucleotide substitution roughly reflected that of the blood samples used to repopulate their mtDNA. (c) 2005 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：255 / 265

页数：11

共 34 条

[1] Detection of mitochondrial DNA depletion in living human cells using PicoGreen staining [J].

Ashley, N ;

Harris, D ;

Poulton, J .

EXPERIMENTAL CELL RESEARCH, 2005, 303 (02) :432-446

[2]

BODNAR AG, 1993, AM J HUM GENET, V53, P663

[3] Mitochondria dysfunction of Alzheimer's disease cybrids enhances Aβ toxicity [J].

Cardoso, SM ;

Santana, I ;

Swerdlow, RH ;

Oliveira, CR .

JOURNAL OF NEUROCHEMISTRY, 2004, 89 (06) :1417-1426

[4]

CHOMYN A, 1994, AM J HUM GENET, V54, P966

[5] The influence of nuclear background on the biochemical expression of 3460 Leber's hereditary optic neuropathy [J].

Cock, HR ;

Tabrizi, SJ ;

Cooper, JM ;

Schapira, AHV .

ANNALS OF NEUROLOGY, 1998, 44 (02) :187-193

[6] DIFFERENT CELLULAR BACKGROUNDS CONFER A MARKED ADVANTAGE TO EITHER MUTANT OR WILD-TYPE MITOCHONDRIAL GENOMES [J].

DUNBAR, DR ;

MOONIE, PA ;

JACOBS, HT ;

HOLT, IJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) :6562-6566

[7]

HARDY M, 1994, J NEUROCHEM, V63, P482

[8] INTRODUCTION OF DISEASE-RELATED MITOCHONDRIAL-DNA DELETIONS INTO HELA-CELLS LACKING MITOCHONDRIAL-DNA RESULTS IN MITOCHONDRIAL DYSFUNCTION [J].

HAYASHI, JI ;

OHTA, S ;

KIKUCHI, A ;

TAKEMITSU, M ;

GOTO, Y ;

NONAKA, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (23) :10614-10618

[9] A novel mitochondrial DNA-like sequence in the human nuclear genome [J].

Herrnstadt, C ;

Clevenger, W ;

Ghosh, SS ;

Anderson, C ;

Fahy, E ;

Miller, S ;

Howell, N ;

Davis, RE .

GENOMICS, 1999, 60 (01) :67-77

[10]

Jun AS, 1996, MOL CELL BIOL, V16, P771

← 1 2 3 4 →