Molecular characterization of mtDNA depleted and repleted NT2 cell lines

被引：20

作者：

Binder, DR ^{[1
]}

Dunn, WH ^{[1
]}

Swerdlow, RH ^{[1
]}

机构：

[1] Univ Virginia, Hlth Syst, Dept Neurol, Charlottesville, VA 22908 USA

来源：

MITOCHONDRION | 2005年 / 5卷 / 04期

关键词：

mitochondrial DNA; NT2; cells; transmitochondrial cybrids;

D O I：

10.1016/j.mito.2005.04.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transmitochondrial cytoplasmic hybrids (cybrids) enable functional assessment of mitochondrial DNA (mtDNA)-encoded proteins. Cybrid production often utilizes cell lines depleted of endogenous mtDNA (p0 cells), and a number of suitable rho 0 cell lines exist for this purpose. We now provide molecular data characterizing an NT2 human teratocarcinoma rho 0 cell line, as well as NT2 cybrid derivatives. NT2 rho 0 cells contained no detectable mtDNA on a sensitive PCR assay. Eight weeks after exogenous mtDNA transfer cybrids showed no evidence of endogenous mtDNA reversion, and heteroplasmic ratios of a single nucleotide substitution roughly reflected that of the blood samples used to repopulate their mtDNA. (c) 2005 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：255 / 265

页数：11

共 34 条

[31] HUMAN NEURONS DERIVED FROM A TERATOCARCINOMA CELL-LINE EXPRESS SOLELY THE 695-AMINO ACID AMYLOID PRECURSOR PROTEIN AND PRODUCE INTRACELLULAR BETA-AMYLOID OR A4 PEPTIDES [J].

WERTKIN, AM ;

TURNER, RS ;

PLEASURE, SJ ;

GOLDE, TE ;

YOUNKIN, SG ;

TROJANOWSKI, JQ ;

LEE, VMY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9513-9517

[32] Differentiation-specific effects of LHON mutations introduced into neuronal NT2 cells [J].

Wong, A ;

Cavelier, L ;

Collins-Schramm, HE ;

Seldin, MF ;

McGrogan, M ;

Savontaus, ML ;

Cortopassi, GA .

HUMAN MOLECULAR GENETICS, 2002, 11 (04) :431-438

[33] MARKED REPLICATIVE ADVANTAGE OF HUMAN MTDNA CARRYING A POINT MUTATION THAT CAUSES THE MELAS ENCEPHALOMYOPATHY [J].

YONEDA, M ;

CHOMYN, A ;

MARTINUZZI, A ;

HURKO, O ;

ATTARDI, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (23) :11164-11168

[34] INDUCIBLE EXPRESSION OF NEURONAL GLUTAMATE RECEPTOR CHANNELS IN THE NT2 HUMAN CELL-LINE [J].

YOUNKIN, DP ;

TANG, CM ;

HARDY, M ;

REDDY, UR ;

SHI, QY ;

PLEASURE, SJ ;

LEE, VMY ;

PLEASURE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (06) :2174-2178

← 1 2 3 4 →