Occurrence and expression of gene transfer agent genes in marine bacterioplankton

被引：71

作者：

Biers, Erin J. ^{[1
]}

Wang, Kui ^{[2
]}

Pennington, Catherine ^{[3
]}

Belas, Robert ^{[2
]}

Chen, Feng ^{[2
]}

Moran, Mary Ann ^{[1
]}

机构：

[1] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA

[2] Univ Maryland, Ctr Marine Biotechnol, Inst Biotechnol, Baltimore, MD 21202 USA

[3] Univ Georgia, Dept Microbiol, Athens, GA 30602 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 10期

关键词：

D O I：

10.1128/AEM.02129-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genes with homology to the transduction-like gene transfer agent (GTA) were observed in genome sequences of three cultured members of the marine Roseobacter clade. A broader search for homologs for this host-controlled virus-like gene transfer system identified likely GTA systems in cultured Alphaproteobacteria, and particularly in marine bacterioplankton representatives. Expression of GTA genes and extracellular release of GTA particles (similar to 50 to 70 nm) was demonstrated experimentally for the Roseobacter clade member Silicibacter pomeroyi DSS-3, and intraspecific gene transfer was documented. GTA homologs are surprisingly infrequent in marine metagenomic sequence data, however, and the role of this lateral gene transfer mechanism in ocean bacterioplankton communities remains unclear.

引用

页码：2933 / 2939

页数：7

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