Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway

被引：71

作者：

Hu, J. -G. ^{[1
,2
,4
]}

Fu, S. -L. ^{[4
]}

Wang, Y. -X. ^{[4
]}

Li, Y. ^{[4
]}

Jiang, X. -Y. ^{[4
]}

Wang, X. -F. ^{[4
]}

Qiu, M. -S. ^{[3
]}

Lu, P. -H. ^{[4
]}

Xu, X. -M. ^{[1
,2
,3
,4
]}

机构：

[1] Indiana Univ, Sch Med, Stark Neurosci Res Inst, Spinal Cord & Brain Injury Res Grp, Indianapolis, IN 46202 USA

[2] Indiana Univ, Sch Med, Dept Neurol Surg, Indianapolis, IN 46202 USA

[3] Univ Louisville, Sch Med, Dept Anat Sci & Neurobiol, Louisville, KY 40292 USA

[4] Shanghai Jiao Tong Univ, Sch Med, Dept Neurobiol, Shanghai 200025, Peoples R China

来源：

NEUROSCIENCE | 2008年 / 151卷 / 01期

关键词：

differentiation; neural stem cells; oligodendrocytes; PDGF-AA; Erk1/2; Olig2;

D O I：

10.1016/j.neuroscience.2007.10.050

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Platelet-derived growth factor-AA (PDGF-AA) has been used as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). Whether it plays a role in oligodendrocyte lineage differentiation of neural stem cells (NSCs) is unclear. Here we report that PDGF-AA is an instructional signal required for the differentiation of embryonic forebrain NSCs into O4-positive oligodendrocytes. Moreover, such PDGF-AA-induced oligodendrocyte differentiation appears to be mediated by the extracellular signal-regulated kinases 1 and 2 (Erk1/2) but not phosphatidylinositol-3 kinase (PI3K) pathway. Finally, PDGF-AA treatment resulted in a significant increase in the expression of the oligodendrocyte-specific transcriptional factor Olig2 in an Erk1/2-dependent mechanism at early stages of oligodendrogliogenesis. Together, our studies provide cellular and molecular evidence to suggest that PDGF-AA is a key molecule that regulates the differentiation of embryonic NSCs into oligodendrocytes. The action of PDGF-AA is mediated by the activation of Erk pathway which involves the downstream upregulation of transcriptional factor Olig2. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：138 / 147

页数：10

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