Lysogenic transfer of group A Streptococcus superantigen gene among streptococci

被引：22

作者：

Vojtek, Ivo ^{[1
]}

Pirzada, Zaid A. ^{[1
]}

Henriques-Normark, Birgitta ^{[2
,3
]}

Mastny, Markus ^{[1
]}

Janapatla, Rajendra P. ^{[1
]}

Charpentier, Emmanuelle ^{[1
]}

机构：

[1] Univ Vienna, Max F Perutz Labs, Dept Microbiol & Immunobiol, A-1030 Vienna, Austria

[2] Swedish Inst Infect Dis Control, Dept Bacteriol, Solna, Sweden

[3] Karolinska Inst, Solna, Sweden

来源：

JOURNAL OF INFECTIOUS DISEASES | 2008年 / 197卷 / 02期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1086/524687

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A groupA Streptococcus(GAS) isolate, serotype M12, recovered from a patient with streptococcal toxic shock syndrome was analyzed for superantigen-carrying prophages, revealing phi 149, which encodes superantigen SSA. Sequence analysis of the att-L proximal region of phi 149 showed that the phage had a mosaic nature. Remarkably, we successfully obtained lysogenic conversion of GAS clinical isolates of various M serotypes (M1, M3, M5, M12, M19, M28, and M94), as well as of group C Streptococcus equisimilis (GCSE) clinical isolates, via transfer of a recombinant phage phi 149::Km(r). Phage phi 149::Km(r) from selected lysogenized GASand GCSE strains could be transferred back to M12 GAS strains. Our data indicate that horizontal transfer of lysogenic phages among GAS can occur across the M-type barrier; these data also provide further support for the hypothesis that toxigenic conversion can occur via lysogeny between species. Streptococci might employ this mechanism specifically to allow more efficient adaptation to changing host challenges, potentially leading to fitter and more virulent clones.

引用

页码：225 / 234

页数：10

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