Synthesis of Laminarin Fragments and Evaluation of a β-(1,3) Glucan Hexasaccaride-CRM197 Conjugate as Vaccine Candidate against Candida albicans

被引：47

作者：

Adamo, Roberto ^{[1
]}

Tontini, Marta ^{[1
]}

Brogioni, Giulia ^{[1
]}

Romano, Maria Rosaria ^{[1
]}

Costantini, Gabriele ^{[1
]}

Danieli, Elisa ^{[1
]}

Proietti, Daniela ^{[1
]}

Berti, Francesco ^{[1
]}

Costantino, Paolo ^{[1
]}

机构：

[1] Novartis Vaccines & Diagnost Res Ctr, I-53100 Siena, Italy

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 2011年 / 30卷 / 4-6期

关键词：

Candida albicans; Glycoconjugate vaccine; Glucans; Delevulinoylation; OLIGOSACCHARIDE CHAIN-LENGTH; HIGHLY EFFICIENT; GLYCOSYLATION; ANTIBODIES; STRATEGY; EPIDEMIOLOGY; DISACCHARIDE; INFECTIONS; PROTECTION; INDUCE;

D O I：

10.1080/07328303.2011.604453

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Laminarin-CRM197 glycoconjugates were previously demonstrated to be immunogenic and confer protection against Candida albicans in mice. Laminarin consists of beta-(1,3) glucan repeating units, with sporadic beta-(1,6) branches. A set of short glucans was used to study the effect of the beta-(1,6) branch on the antigenicity of linear beta-(1,3) glucans. A linear beta-(1,3) glucan hexasaccharide was selected as the best fragment able to inhibit the binding between laminarin and antilaminarin antibodies. The hexamer was then conjugated to CRM197 and induced, in mice, significant titers of specific antilaminarin antibodies comparable with those raised by the Lam-CRM197 conjugate.

引用

页码：249 / 280

页数：32

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