Detection of NO release from endothelial cells using Pt micro electrodes modified with a pyrrole-functionalised Mn(II) porphyrin

被引：19

作者：

Oni, J ^{[1
]}

Diab, N ^{[1
]}

Radtke, I ^{[1
]}

Schuhmann, W ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Lehrstuhl Mol Neurobiochem, D-44780 Bochum, Germany

来源：

ELECTROCHIMICA ACTA | 2003年 / 48卷 / 20-22期

关键词：

nitric oxide; manganese porphyrin; human umbilical vein endothelial cells; endothelial cell; conducting polymer; microsensor; modified electrode;

D O I：

10.1016/S0013-4686(03)00404-3

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Pt-disk electrodes (50 mum diameter) were modified with pyrrole-functionalised Mn(II) trimethoxycarboxyphenylporphyrin by means of an electrochemical pulse technique leading to stable and sensitive microsensors that displayed electrocatalytic activitiy suitable for the detection and quantification of nitric oxide (NO). In order to improve selectivity, a thin layer of Nafion(R) was deposited on top of the porphyrin-modified surface of the microsensor by means of a dip-coating procedure. These microelectrodes were successfully applied for the detection of the secretion of NO from a population of human umbilical vein endothelial cells (HUVEC) into a small volume of electrolyte. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：3349 / 3354

页数：6

共 40 条

[1]

Beckman Joseph S., 1995, Methods (Orlando), V7, P35, DOI 10.1006/meth.1995.1005

[2] Design and characterization of chemically modified electrodes with iron(III) porphyrinic-based polymers: Study of their reactivity toward nitrites and nitric oxide in aqueous solution [J].

Bedioui, F ;

Trevin, S ;

Albin, V ;

Villegas, MGG ;

Devynck, J .

ANALYTICA CHIMICA ACTA, 1997, 341 (2-3) :177-185

[3] Porphyrins in analytical chemistry. A review [J].

Biesaga, M ;

Pyrzynska, K ;

Trojanowicz, M .

TALANTA, 2000, 51 (02) :209-224

[4] NANOGRAM NITRITE AND NITRATE DETERMINATION IN ENVIRONMENTAL AND BIOLOGICAL-MATERIALS BY VANADIUM(III) REDUCTION WITH CHEMI-LUMINESCENCE DETECTION [J].

BRAMAN, RS ;

HENDRIX, SA .

ANALYTICAL CHEMISTRY, 1989, 61 (24) :2715-2718

[5] THE PHYSIOLOGICAL-ROLE OF NITRIC-OXIDE [J].

BUTLER, AR ;

WILLIAMS, DLH .

CHEMICAL SOCIETY REVIEWS, 1993, 22 (04) :233-241

[6] The role of nickel as central metal in conductive polymeric porphyrin film for electrocatalytic oxidation of nitric oxide [J].

Ciszewski, A ;

Kubaszewski, E ;

Lozynski, M .

ELECTROANALYSIS, 1996, 8 (03) :293-295

[7] Electropolymerized manganese porphyrin/polypyrrole films as catalytic surfaces for the oxidation of nitric oxide [J].

Diab, N ;

Schuhmann, W .

ELECTROCHIMICA ACTA, 2001, 47 (1-2) :265-273

[8] IMPAIRMENT OF ENDOTHELIUM-DEPENDENT RELAXATION IN AORTAE FROM SPONTANEOUSLY DIABETIC RATS [J].

DURANTE, W ;

SEN, AK ;

SUNAHARA, FA .

BRITISH JOURNAL OF PHARMACOLOGY, 1988, 94 (02) :463-468

[9] Voltammetric detection of NO in the rat brain with an electronic conducting polymer and Nafion(R) bilayer-coated carbon fibre electrode [J].

Fabre, B ;

Burlet, S ;

Cespuglio, R ;

Bidan, G .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 426 (1-2) :75-83

[10] SPECTROFLUOROMETRIC METHOD FOR NO DETERMINATION [J].

GABOR, G ;

ALLON, N .

ANALYTICAL BIOCHEMISTRY, 1994, 220 (01) :16-19

← 1 2 3 4 →