Inheritance of Arabidopsis DNA in offspring from Brassica napus and A-thaliana somatic hybrids

被引:7
作者
Bohman, S [1 ]
Forsberg, J [1 ]
Glimelius, K [1 ]
Dixelius, C [1 ]
机构
[1] Swedish Univ Agr Sci, Uppsala Genet Ctr, Dept Plant Biol, S-75007 Uppsala, Sweden
关键词
Brassicaceae; back-crossed symmetric and asymmetric somatic hybrids; RFLP analysis; chromosomes; intergenomic translocation;
D O I
10.1007/s001220051045
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Chromosome counts and RFLP markers mapped to Arabidopsis thaliana were used to determine the proportion of eliminated chromosomes and retained A. thaliana DNA in the back-crossed (BC) progeny derived from symmetric and asymmetric somatic hybrids between Brassica napus and A. thaliana. All plants were analysed for the presence of two RFLP markers per chromosome, preferably with one located on each chromosome arm. A reduction in both A. thaliana RFLP markers and chromosome numbers was found in the BC1 and BC2 generations of the symmetric hybrids as well as in the BC1 generation of the asymmetric hybrids. In the symmetric hybrids, two back-crosses to B. napus were required to reduce the frequency of retained A. thaliana loci to 42.4% and mean chromosome number to 39.4. In comparison, the BC1 progeny of the asymmetric hybrids had 16% of the analysed A. thaliana loci present and an average of 38.4 chromosomes maintained. When the frequency of A. thaliana chromosomes with both analysed loci maintained was compared with the frequency of chromosomes with one locus lost and one kept, a reduction in the number of complete chromosomes between BC1 and BC2 derived from the symmetric hybrids was observed. Among the BC1 plants in the asymmetric group the situation was different, with higher amounts of incomplete donor chromosomes compared to whole chromosomes. The results indicate that A. thaliana chromosome fragments are more often found in the progeny of irradiated hybrids, while back-crossed symmetric hybrids have more complete chromosomes.
引用
收藏
页码:99 / 106
页数:8
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