Detection of rat Pneumocystis carinii proteinases and elastase and antipneumocystis activity of proteinase inhibitors in vitro

被引:7
作者
Atzori, C
Mainini, A
Agostoni, F
Angeli, E
Bartlett, M
Bruno, A
Scaglia, M
Cargnel, A
机构
[1] L Sacco Hosp Vialba, Div Infect Dis 2, Milan, Italy
[2] Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN USA
[3] Policlin San Matteo, IRCCS, Parasitol Lab, Pavia, Italy
关键词
P-carinii proteinases; elastase; proteinase inhibitors;
D O I
10.1051/parasite/1999061009
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Proteinases play an important role in survival of microorganisms and in pathogenicity of diseases. By using a modified SDS-gelatin-polyacrylamide gel system, proteinases of rat-P.carinii were detected as bonds of proteolytic digestion after electrophoresis. P.carinii organisms obtained from dexamethazone immunosuppressed transtracheally infected rots were cultured in spinner Flask suspension cultures to minimize host cell contamination. At pH 8.3, seven Pc-specific proteolytic bands were detected in three clusters of different molecular weights clearly different from host cell patterns. By using a range of pH, various preparations of organisms and bath infected and uninfected culture media, proteolytic activities have been partially characterized. Elastase secretion has been assessed based on elastin digestion model. Proteinase inhibitors have been tested for their ability to inhibit P.carinii growth in HEL299 short-term monolayer cultures. Results indicate that proteolytic activities are involved in the proliferation of microorganisms since leupeptin exerted in vitro antipneumocystis activity while aprotinin enhanced P.carinii growth.
引用
收藏
页码:9 / 16
页数:8
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