Expression of γ-tocopherol methyltransferase gene from Brassica napus increased α-tocopherol content in soybean seed

被引:23
作者
Chen, D. F. [1 ]
Zhang, M. [1 ]
Wang, Y. Q. [1 ]
Chen, X. W. [1 ]
机构
[1] Nankai Univ, Mol Genet Lab, Coll Life Sci, Tianjin 300071, Peoples R China
基金
中国国家自然科学基金;
关键词
fatty acid elongase 1; Glycine max; glycinin; RT-PCR; Southern blot; transgenic plants; VITAMIN-E CONTENT; TMT GENE; TRANSFORMATION; PLANTS;
D O I
10.1007/s10535-012-0028-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A cDNA encoding gamma-tocopherol methyltransferase from Brassica napus (BnTMT) was overexpressed in soybean [Glycine max (L.) Merr.] under the control of seed-specific promoter of Arabidopsis fatty acid elongase 1 (FAE1) or soybean glycinin G1. Two and three transgenic plants were selected, respectively, after Agrobacterium-mediated transformation. Polymerase chain reaction (PCR) and Southern blots confirmed that BnTMT was single-copy integrated into the genome of transgenic plants. RT-PCR analysis showed that the expression of BnTMT was higher in the immature cotyledons than in the mature cotyledons, while no expression was detected in the leaves. Moreover, the expression level under the control of FAE1 was higher than that of G1. HPLC analysis indicated that the seed-specific expression of BnTMT resulted in 11.1-fold and 18.9-fold increase in alpha- and beta-tocopherol content, respectively, in T-2 seed. These results suggested that introducing BnTMT into soybean can be used to increase the vitamin E composition in seeds.
引用
收藏
页码:131 / 134
页数:4
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