Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases:: Biological implications for cell wall metabolism

被引:213
作者
Baumann, Martin J.
Eklof, Jens M.
Michel, Gurvan
Kallas, Asa M.
Teeri, Tuula T.
Czjzek, Mirjam [1 ]
Brumer, Harry, III
机构
[1] Univ Paris 06, Equipe Glycobiol Marine, UMR 7139, CNRS,Stn Biol, F-29682 Roscoff, France
[2] AlbaNova Univ Ctr, Royal Inst Technol, Sch Biotechnol, S-10691 Stockholm, Sweden
关键词
D O I
10.1105/tpc.107.051391
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen ( Populus tremula 3 Populus tremuloides). Production of the loop deletion variant Tm-NXG1-Delta YNIIG yielded an enzyme that was structurally similar to Ptt- XET16-34 and had a greatly increased transglycosylation: hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.
引用
收藏
页码:1947 / 1963
页数:17
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