Proteomic profiling using mass spectrometry does normalising by total ion current potentially mask some biological differences?

被引：25

作者：

Cairns, David A. ^{[1
]}

Thompson, Douglas ^{[1
,2
]}

Perkins, David N. ^{[1
]}

Stanley, Anthea J. ^{[1
]}

Selby, Peter J. ^{[1
]}

Banks, Rosamonde E. ^{[1
]}

机构：

[1] St James Univ Hosp, Canc Res UK Clin Ctr, Leeds Inst Mol Med, Clin & Biomed Proteom Grp, Leeds LS9 7TF, W Yorkshire, England

[2] Leeds Gen Infirm, Dept Clin Biochem & Immunol, Leeds, W Yorkshire, England

来源：

PROTEOMICS | 2008年 / 8卷 / 01期

关键词：

MALDI; normalisation; SELDI; total ion current;

D O I：

10.1002/pmic.200700598

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Normalisation of data to minimise the impact of technical variation on comparative sample analysis is often carried out. Using SELDI data as a model, we have examined the effects of normalisation by TIC which is commonly used for MS data. Significant intergroup differences in normalisation factor were found for serum profiles which could not be explained by experimental factors, implying that normalisation by TIC may in some situations also normalise biological differences and should be systematically evaluated.

引用

页码：21 / 27

页数：7

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