Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread

被引：79

作者：

Celle, Olivier ^{[1
]}

Blanchard, Philippe ^{[1
]}

Olivier, Violaine ^{[1
]}

Schuff, Frank ^{[1
]}

Cougoule, Nicolas ^{[1
]}

Faucon, Jean-Paul ^{[1
]}

Ribiere, Magali ^{[1
]}

机构：

[1] AFSSA Sophia Antipolis, Unite Pathol Abeille, F-06902 Sophia Antipolis, France

来源：

VIRUS RESEARCH | 2008年 / 133卷 / 02期

关键词：

Chronic bee paralysis virus (CBPV); Camponotus vagus; Formica rufa; Varroa destructor; quantitation; minus-strand-specific RT-PCR; Apis mellifera;

D O I：

10.1016/j.virusres.2007.12.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：280 / 284

页数：5

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