Dendrigraft polylysine coated-poly(glycolic acid) fibrous scaffolds for hippocampal neurons

被引：15

作者：

Kojima, Chie ^{[1
]}

Fusaoka-Nishioka, Eri ^{[2
]}

Imai, Toshio ^{[2
]}

Nakahira, Atsushi ^{[3
]}

Onodera, Hiroshi ^{[4
,5
]}

机构：

[1] Osaka Prefecture Univ, Grad Sch Engn, Dept Appl Chem, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan

[2] KAN Res Inst, Dept Adv Biomed, Chuo Ku, 6-8-2 Minatojima Minamimachi, Kobe, Hyogo 6500047, Japan

[3] Osaka Prefecture Univ, Grad Sch Engn, Dept Mat Sci, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan

[4] Univ Tokyo, Photon Sci Ctr, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan

[5] Univ Tokyo, Ctr Innovat, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2016年 / 104卷 / 11期

基金：

日本科学技术振兴机构;

关键词：

polylysine; neuron culture; dendrimer; dendrigraft; poly(glycolic acid); PERIPHERAL-NERVE REGENERATION; DENDRITIC POLY(L-LYSINE); SURFACE-CHEMISTRY; STEM-CELLS; IN-VITRO; DENDRIMERS; GROWTH; PROLIFERATION; ATTACHMENT; POLYMERS;

D O I：

10.1002/jbm.a.35807

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Poly(glycolic acid) (PGA) fibers are a good candidate material for nerve cell scaffolds, which is applicable to the treatment of peripheral nerve injuries. Polylysine is widely used as a coating material for cell substrates to promote nerve cell adhesion. In this study, linear and dendrigraft polylysines were used to coat PGA fibers. The association of large dendrigraft polylysines with PGA fibers was lower and unstable, compared with linear polylysine. However, more hippocampal neurons adhered to PGA fibers coated with large dendrigraft polylysine than linear polylysine. Enhanced cell adhesion was observed, even when the dendrigraft polylysine was coated on the PGA fibers at a low concentration (0.05 mu g/mL) or when it was coated in water instead of alkaline buffer. Differences in cell adhesion properties were seen between the dendrigraft polylysine coating and a laminin coating. Thus, large dendrigraft polylysines are a useful coating material for nerve cell scaffolds. (C) 2016 Wiley Periodicals, Inc.

引用

页码：2744 / 2750

页数：7

共 33 条

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